Shestakova E A, Motuz L P, Gavrilova L P
Institute of Protein Research, Academy of Sciences of Russia, Pushchino, Moscow Region.
Cell Biol Int. 1993 Apr;17(4):417-24. doi: 10.1006/cbir.1993.1080.
The distribution of eukaryotic elongation factor 2 (eEF-2) in G0-arrested fixed human skin diploid fibroblasts was studied by indirect immunofluorescent microscopy. It was found earlier that the main part of eEF-2 in cycling cells was located near the nucleus in the endoplasm (Gavrilova et al., 1987). It has been demonstrated here that the transition from proliferation to the G0 phase of the cell cycle leads to the distribution of eEF-2 mainly along the intermediate filaments and/or microtubules. Both in cycling and in G0-arrested fibroblasts a portion of eEF-2 is also co-localized with actin microfilament bundles. The reversion of the cells from the G0 phase to proliferation is accompanied by rearrangement of the actin cytoskeleton and reversal to the original pattern of eEF-2 distribution. It is likely that the different types of cytoskeleton in eukaryotic cells can be involved in organization of protein-synthesizing machinery.
采用间接免疫荧光显微镜技术研究了真核生物延伸因子2（eEF - 2）在G0期停滞的固定人皮肤二倍体成纤维细胞中的分布。先前发现，处于细胞周期的增殖细胞中，eEF - 2的主要部分位于内质网中靠近细胞核的位置（加夫里洛娃等人，1987年）。本文已证明，细胞周期从增殖期向G0期的转变导致eEF - 2主要沿中间丝和/或微管分布。在增殖的和成G0期停滞的成纤维细胞中，一部分eEF - 2也与肌动蛋白微丝束共定位。细胞从G0期恢复到增殖状态伴随着肌动蛋白细胞骨架的重排以及eEF - 2分布模式恢复到原始状态。真核细胞中不同类型的细胞骨架可能参与了蛋白质合成机制的组织。
