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真核起始因子（iso）4F的p86亚基作为植物细胞中微管相关蛋白的功能。

Function of the p86 subunit of eukaryotic initiation factor (iso)4F as a microtubule-associated protein in plant cells.

作者信息

Bokros C L, Hugdahl J D, Kim H H, Hanesworth V R, van Heerden A, Browning K S, Morejohn L C

机构信息

Department of Botany, University of Texas, Austin 78713, USA.

出版信息

Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):7120-4. doi: 10.1073/pnas.92.15.7120.

DOI:10.1073/pnas.92.15.7120
PMID:7624381
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC41483/
Abstract

The isozyme form of eukaryotic initiation factor 4F [eIF-(iso)4F] from wheat germ is composed of a p28 subunit that binds the 7-methylguanine cap of mRNA and a p86 subunit having unknown function. The p86 subunit was found to have limited sequence similarity to a kinesin-like protein encoded by the katA gene of Arabidopsis thaliana. Native wheat germ eIF-(iso)4F and bacterially expressed p86 subunit and p86-p28 complex bound to taxol-stabilized maize microtubules (MTs) in vitro. Binding saturation occurred at 1 mol of p86 per 5-6 mol of polymerized tubulin dimer, demonstrating a substoichiometric interaction of p86 with MTs. No evidence was found for a direct interaction of the p28 subunit with MTs. Unlike kinesin, cosedimentation of eIF-(iso)4F with MTs was neither reduced by MgATP nor enhanced by adenosine 5'-[gamma-imido]triphosphate. Both p86 subunit and p86-p28 complex induced the bundling of MTs in vitro. The p86 subunit was immunolocalized to the cytosol in root maize cells and existed in three forms: fine particles, coarse particles, and linear patches. Many coarse particles and linear patches were colocalized or closely associated with cortical MT bundles in interphase cells. The results indicate that the p86 subunit of eIF-(iso)4F is a MT-associated protein that may simultaneously link the translational machinery to the cytoskeleton and regulate MT disposition in plant cells.

摘要

来自小麦胚芽的真核生物起始因子4F [eIF-(iso)4F]的同工酶形式由一个结合mRNA的7-甲基鸟嘌呤帽的p28亚基和一个功能未知的p86亚基组成。发现p86亚基与拟南芥katA基因编码的一种驱动蛋白样蛋白具有有限的序列相似性。天然小麦胚芽eIF-(iso)4F以及细菌表达的p86亚基和p86-p28复合物在体外与紫杉醇稳定的玉米微管（MTs）结合。结合饱和度在每5 - 6摩尔聚合微管蛋白二聚体中有1摩尔p86时出现，表明p86与MTs之间存在亚化学计量的相互作用。未发现p28亚基与MTs直接相互作用的证据。与驱动蛋白不同，eIF-(iso)4F与MTs的共沉降既不会被MgATP降低，也不会被腺苷5'-[γ-亚氨基]三磷酸增强。p86亚基和p86-p28复合物在体外都能诱导MTs成束。p86亚基在玉米根细胞的细胞质中被免疫定位，并且以三种形式存在：细颗粒、粗颗粒和线性斑块。在间期细胞中，许多粗颗粒和线性斑块与皮层MT束共定位或紧密相关。结果表明，eIF-(iso)4F的p86亚基是一种与MT相关的蛋白，它可能同时将翻译机制与细胞骨架连接起来，并调节植物细胞中MT的分布。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/864184f61d2e/pnas01491-0479-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/91ebebcf5ec7/pnas01491-0477-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/d2e176d34dee/pnas01491-0478-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/1f3525c491fd/pnas01491-0478-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/6950710080e7/pnas01491-0479-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/5e5c50d32ea2/pnas01491-0479-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/864184f61d2e/pnas01491-0479-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/91ebebcf5ec7/pnas01491-0477-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/d2e176d34dee/pnas01491-0478-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/1f3525c491fd/pnas01491-0478-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/6950710080e7/pnas01491-0479-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/5e5c50d32ea2/pnas01491-0479-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/28de/41483/864184f61d2e/pnas01491-0479-c.jpg

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2
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3
Silver-enhanced copper staining of protein blots.
BMC Biochem. 2008 Sep 15;9:23. doi: 10.1186/1471-2091-9-23.
4
The structure of eukaryotic translation initiation factor-4E from wheat reveals a novel disulfide bond.来自小麦的真核生物翻译起始因子4E的结构揭示了一种新的二硫键。
Plant Physiol. 2007 Apr;143(4):1504-18. doi: 10.1104/pp.106.093146. Epub 2007 Feb 23.
5
Tobacco mosaic virus movement protein functions as a structural microtubule-associated protein.烟草花叶病毒运动蛋白作为一种与微管相关的结构蛋白发挥作用。
J Virol. 2006 Sep;80(17):8329-44. doi: 10.1128/JVI.00540-06.
6
Cap-independent translation of plant viral RNAs.植物病毒RNA的非帽依赖性翻译
Virus Res. 2006 Jul;119(1):63-75. doi: 10.1016/j.virusres.2005.10.010. Epub 2005 Dec 19.
7
Temporal and spatial expression patterns of TUB9, a beta-tubulin gene of Arabidopsis thaliana.拟南芥β-微管蛋白基因TUB9的时空表达模式
Plant Mol Biol. 2001 Oct;47(3):389-98. doi: 10.1023/a:1011628024798.
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