Sporik R B, Arruda L K, Woodfolk J, Chapman M D, Platts-Mills T A
Division of Allergy and Clinical Immunology, University of Virginia Health Sciences Center, Charlottesville 22908.
Clin Exp Allergy. 1993 Apr;23(4):326-31. doi: 10.1111/j.1365-2222.1993.tb00330.x.
Asp f I is a major allergen produced by the mycelia of Aspergillus fumigatus. It is not present in spores and can be used as a specific marker for the detection of germination of this fungus. We investigated the domestic and outdoor concentration of Asp f I in Poole, U.K. and Charlottesville, VA, U.S.A. Asp f I was undetectable in 95% (281/296) of house dust extracts and present at low levels (< 0.17 micrograms/g of sieved dust, mean 0.038 micrograms/g) in the remainder. In contrast, Asp fI could be detected in 65% (15/23) of cultures of house dust, suggesting the presence of viable, but ungerminated, A. fumigatus in the majority of homes. Asp f I was detectable in 80% (28/35) of extracts of leaves and compost, but present in these outdoor samples at low levels (< 0.11 micrograms/g, mean 0.27 micrograms/g). Air sampling for Asp f I was undertaken before and after vigorous disturbances at indoor (n = 5) and outdoor (n = 6) sites. Airborne Asp f I was not detected in domestic samples or in undisturbed outdoor samples. Following disturbance it could be measured in outdoor samples (range 7.6-29 ng/m3). The results suggest that while exposure to A. fumigatus is common, exposure to Asp f I and germinating A. fumigatus is uncommon. It is probable that those individuals who develop antibody responses to Asp f I have been exposed to A. fumigatus which has germinated in their respiratory tract.
烟曲霉Asp f I是烟曲霉菌丝体产生的一种主要变应原。它不存在于孢子中，可作为检测该真菌萌发的特异性标志物。我们调查了英国普尔和美国弗吉尼亚州夏洛茨维尔室内和室外环境中Asp f I的浓度。在95%（281/296）的房屋灰尘提取物中未检测到Asp f I，其余提取物中含量较低（<0.17微克/克过筛灰尘，平均0.038微克/克）。相比之下，在65%（15/23）的房屋灰尘培养物中可检测到Asp fI，这表明大多数家庭中存在存活但未萌发的烟曲霉。在80%（28/35）的树叶和堆肥提取物中可检测到Asp f I，但在这些室外样本中的含量较低（<0.11微克/克，平均0.27微克/克）。在室内（n = 5）和室外（n = 6）场所进行剧烈扰动前后对Asp f I进行空气采样。在家庭样本或未受扰动的室外样本中未检测到空气中的Asp f I。扰动后，可在室外样本中检测到（范围为7.6 - 29纳克/立方米）。结果表明，虽然接触烟曲霉很常见，但接触Asp f I和萌发的烟曲霉并不常见。那些对Asp f I产生抗体反应的个体很可能接触过在其呼吸道中萌发的烟曲霉。
