• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

两种新型多用途多拷贝粟酒裂殖酵母穿梭载体，pSP1和pSP2。

Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2.

作者信息

Cottarel G, Beach D, Deuschle U

机构信息

Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, NY 11724.

出版信息

Curr Genet. 1993 May-Jun;23(5-6):547-8. doi: 10.1007/BF00312650.

DOI:10.1007/BF00312650
PMID:8319314
Abstract

Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly.

摘要

质粒pSP1和pSP2是两种新的粟酒裂殖酵母ars1多拷贝载体，以酿酒酵母LEU2和URA3基因作为选择标记。它们是酿酒酵母整合质粒的衍生物。这些质粒使得经典分子遗传学技术，如诱变、嵌套缺失和测序，能够直接进行。

相似文献

1
Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2.两种新型多用途多拷贝粟酒裂殖酵母穿梭载体，pSP1和pSP2。
Curr Genet. 1993 May-Jun;23(5-6):547-8. doi: 10.1007/BF00312650.
2
The Saccharomyces cerevisiae HIS3 and LYS2 genes complement the Schizosaccharomyces pombe his5-303 and lys1-131 mutations, respectively: new selectable markers and new multi-purpose multicopy shuttle vectors, pSP3 and pSP4.酿酒酵母HIS3和LYS2基因分别互补粟酒裂殖酵母his5 - 303和lys1 - 131突变：新的选择标记及新的多用途多拷贝穿梭载体pSP3和pSP4。
Curr Genet. 1995 Sep;28(4):380-3. doi: 10.1007/BF00326437.
3
Versatile use of Schizosaccharomyces pombe plasmids in Saccharomyces cerevisiae.酿酒酵母中裂殖酵母质粒细胞系质粒的多功能用途。
FEMS Yeast Res. 2011 Dec;11(8):653-5. doi: 10.1111/j.1567-1364.2011.00752.x. Epub 2011 Sep 15.
4
A family of multifunctional thiamine-repressible expression vectors for fission yeast.用于裂殖酵母的多功能硫胺素可抑制表达载体家族。
Yeast. 2000 Jun 30;16(9):861-72. doi: 10.1002/1097-0061(20000630)16:9<861::AID-YEA577>3.0.CO;2-9.
5
Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe.用于粟酒裂殖酵母的多功能穿梭载体和基因组文库。
Gene. 1992 May 1;114(1):59-66. doi: 10.1016/0378-1119(92)90707-v.
6
Vectors for the construction of gene banks and the integration of cloned genes in Schizosaccharomyces pombe and Saccharomyces cerevisiae.用于构建基因文库以及将克隆基因整合到粟酒裂殖酵母和酿酒酵母中的载体。
Plasmid. 1986 Mar;15(2):156-8. doi: 10.1016/0147-619x(86)90051-x.
7
Construction of Designer Selectable Marker Deletions with a CRISPR-Cas9 Toolbox in and New Design of Common Entry Vectors.利用CRISPR-Cas9工具盒构建特定设计的可选择标记缺失以及通用入门载体的新设计。
G3 (Bethesda). 2018 Mar 2;8(3):789-796. doi: 10.1534/g3.117.300363.
8
Co-expression of a Saccharomyces diastaticus glucoamylase-encoding gene and a Bacillus amyloliquefaciens alpha-amylase-encoding gene in Saccharomyces cerevisiae.糖化酵母葡糖淀粉酶编码基因与解淀粉芽孢杆菌α-淀粉酶编码基因在酿酒酵母中的共表达。
Gene. 1991 Apr;100:85-93. doi: 10.1016/0378-1119(91)90353-d.
9
Cloning the Schizosaccharomyces pombe lys2+ gene and construction of new molecular genetic tools.粟酒裂殖酵母lys2+基因的克隆及新型分子遗传工具的构建。
Curr Genet. 2006 Jun;49(6):414-20. doi: 10.1007/s00294-006-0065-2. Epub 2006 Feb 21.
10
Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe.用于粟酒裂殖酵母his3缺陷菌株的载体构建及基因组文库构建。
Gene. 1996 Oct 3;174(2):315-8. doi: 10.1016/0378-1119(96)00085-6.

引用本文的文献

1
The heme-regulated inhibitor kinase Hri1 is activated in response to aminolevulinic acid deficiency in Schizosaccharomyces pombe.血红素调节抑制剂激酶Hri1在粟酒裂殖酵母中对δ-氨基乙酰丙酸缺乏作出反应时被激活。
PLoS Genet. 2025 Jul 16;21(7):e1011797. doi: 10.1371/journal.pgen.1011797. eCollection 2025 Jul.
2
The ornithine-N-oxygenase Sib2 interacts with the N-transacetylase Sib3 in the ferrichrome biosynthetic pathway.鸟氨酸-N-加氧酶Sib2在高铁载体生物合成途径中与N-转乙酰酶Sib3相互作用。
Front Microbiol. 2024 Sep 10;15:1467397. doi: 10.3389/fmicb.2024.1467397. eCollection 2024.
3
Nucleotide exchange is sufficient for Hsp90 functions in vivo.

本文引用的文献

1
Replication and recombination of 2-µm DNA in Schizosaccharomyces pombe.酿酒酵母 2μm 质粒 DNA 的复制和重组。
Curr Genet. 1983 Jul;7(4):245-53. doi: 10.1007/BF00376069.
2
High-frequency transformation of the fission yeast Schizosaccharomyces pombe.高频转化裂殖酵母酿酒酵母。
Nature. 1981 Mar 12;290(5802):140-2. doi: 10.1038/290140a0.
3
Functionally homologous cell cycle control genes in budding and fission yeast.芽殖酵母和裂殖酵母中功能同源的细胞周期调控基因。
核苷酸交换足以满足 Hsp90 在体内的功能。
Nat Commun. 2023 Apr 29;14(1):2489. doi: 10.1038/s41467-023-38230-0.
4
Sib1, Sib2, and Sib3 proteins are required for ferrichrome-mediated cross-feeding interaction between and .Sib1、Sib2和Sib3蛋白是铁载体介导的[具体对象1]与[具体对象2]之间交叉喂养相互作用所必需的。
Front Microbiol. 2022 Jul 19;13:962853. doi: 10.3389/fmicb.2022.962853. eCollection 2022.
5
Pericentromere-Specific Cohesin Complex Prevents Meiotic Pericentric DNA Double-Strand Breaks and Lethal Crossovers.着丝粒特异性黏合复合物防止减数分裂着丝粒区 DNA 双链断裂和致死性交叉。
Mol Cell. 2018 Aug 16;71(4):540-553.e4. doi: 10.1016/j.molcel.2018.06.035. Epub 2018 Aug 2.
6
Construction of Designer Selectable Marker Deletions with a CRISPR-Cas9 Toolbox in and New Design of Common Entry Vectors.利用CRISPR-Cas9工具盒构建特定设计的可选择标记缺失以及通用入门载体的新设计。
G3 (Bethesda). 2018 Mar 2;8(3):789-796. doi: 10.1534/g3.117.300363.
7
Kinetochore Components Required for Centromeric Chromatin Assembly Are Impacted by Msc1 in .着丝粒染色质组装所需的动粒组件受Msc1的影响 。 （原句结尾不完整，翻译可能会有一定局限性）
Genetics. 2017 Oct;207(2):559-569. doi: 10.1534/genetics.117.300183. Epub 2017 Aug 21.
8
Development of a pyrE-based selective system for Thermotoga sp. strain RQ7.嗜热栖热菌属菌株RQ7基于pyrE的选择系统的开发。
Extremophiles. 2017 Mar;21(2):297-306. doi: 10.1007/s00792-016-0902-2. Epub 2016 Dec 7.
9
Cwf16p Associating with the Nineteen Complex Ensures Ordered Exon Joining in Constitutive Pre-mRNA Splicing in Fission Yeast.Cwf16p与19S复合体结合确保裂殖酵母组成型前体mRNA剪接中有序的外显子连接。
PLoS One. 2015 Aug 24;10(8):e0136336. doi: 10.1371/journal.pone.0136336. eCollection 2015.
10
Mfc1 is a novel forespore membrane copper transporter in meiotic and sporulating cells.Mfc1 是减数分裂和孢子形成细胞中新的前孢子膜铜转运蛋白。
J Biol Chem. 2011 Sep 30;286(39):34356-72. doi: 10.1074/jbc.M111.280396. Epub 2011 Aug 2.
Nature. 1982 Dec 23;300(5894):706-9. doi: 10.1038/300706a0.
4
Plasmids carrying the yeast OMP decarboxylase structural and regulatory genes: transcription regulation in a foreign environment.携带酵母OMP脱羧酶结构基因和调控基因的质粒：在异源环境中的转录调控
Cell. 1983 Feb;32(2):371-7. doi: 10.1016/0092-8674(83)90456-7.
5
Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation.利用酵母细菌穿梭载体构建粟酒裂殖酵母基因文库及其通过互补作用分离基因的应用。
Mol Gen Genet. 1982;187(2):326-9. doi: 10.1007/BF00331138.
6
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.用核酸外切酶III进行单向消化可为DNA测序创建靶向断点。
Gene. 1984 Jun;28(3):351-9. doi: 10.1016/0378-1119(84)90153-7.
7
Sequence analysis of ARS elements in fission yeast.裂殖酵母中自主复制序列元件的序列分析。
EMBO J. 1988 Jul;7(7):2203-9. doi: 10.1002/j.1460-2075.1988.tb03059.x.
8
Sucl+ encodes a predicted 13-kilodalton protein that is essential for cell viability and is directly involved in the division cycle of Schizosaccharomyces pombe.
Mol Cell Biol. 1987 Jan;7(1):504-11. doi: 10.1128/mcb.7.1.504-511.1987.
9
Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element.在粟酒裂殖酵母中复制质粒：一种新的遗传元件对对称分离的改善
Mol Cell Biol. 1986 Jan;6(1):80-9. doi: 10.1128/mcb.6.1.80-89.1986.
10
Vectors for the construction of gene banks and the integration of cloned genes in Schizosaccharomyces pombe and Saccharomyces cerevisiae.用于构建基因文库以及将克隆基因整合到粟酒裂殖酵母和酿酒酵母中的载体。
Plasmid. 1986 Mar;15(2):156-8. doi: 10.1016/0147-619x(86)90051-x.