两种新型多用途多拷贝粟酒裂殖酵母穿梭载体，pSP1和pSP2。
Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2.
作者信息
Cottarel G, Beach D, Deuschle U
机构信息
Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, NY 11724.
出版信息
Curr Genet. 1993 May-Jun;23(5-6):547-8. doi: 10.1007/BF00312650.
PMID:8319314
Abstract
Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly.
摘要
质粒pSP1和pSP2是两种新的粟酒裂殖酵母ars1多拷贝载体，以酿酒酵母LEU2和URA3基因作为选择标记。它们是酿酒酵母整合质粒的衍生物。这些质粒使得经典分子遗传学技术，如诱变、嵌套缺失和测序，能够直接进行。
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