Vespa M N, Lebecq J C, Simonetti N, Bastide J M
Istituto di Microbiologia, Facoltà di Farmacia, Università degli Studi di Roma, La Sapienza, Italy.
Comp Immunol Microbiol Infect Dis. 1993 Apr;16(2):163-72. doi: 10.1016/0147-9571(93)90009-t.
We report on the effect of subinhibitory doses of tunicamycin on Candida albicans cells (BP strain high responder NCYC 1466) in a defined medium favourable for expression of the mycelial phase. Tunicamycin inhibited the synthesis of some protein fractions ranging from 40 to 65 kDa, where the immunodominant antigens of C. albicans responsible for the antibody response to systemic mycosis were inhibited. By two-dimensional immunoelectrophoresis, antigen extracts from the cell cultures grown with tunicamycin showed a migration modification and a lower number of precipitation arcs with variation in their height and range.
我们报道了在有利于菌丝相表达的特定培养基中，亚抑制剂量的衣霉素对白色念珠菌细胞（BP菌株高反应性NCYC 1466）的影响。衣霉素抑制了一些分子量在40至65 kDa之间的蛋白质组分的合成，其中负责全身性真菌病抗体反应的白色念珠菌免疫显性抗原受到抑制。通过二维免疫电泳，用衣霉素培养的细胞培养物中的抗原提取物显示出迁移改变，沉淀弧数量减少，其高度和范围也有所变化。
