Chaffin W L
J Gen Microbiol. 1985 Aug;131(8):1853-61. doi: 10.1099/00221287-131-8-1853.
Tunicamycin is an antimicrobial agent which inhibits the first reaction of the dolichol pathway leading to N-glycosylation of proteins. The effect of tunicamycin on the growth of the dimorphic fungus Candida albicans differed depending on the growth phase of the organism. Addition of tunicamycin to stationary phase yeast cells inhibited the resumption of growth of those cells in either morphology, as cultures failed to initiate either yeast bud or germ tube formation. When tunicamycin was added to growing cells, growth was inhibited but not immediately. When it was added to germ tube cultures, nuclear division and septum formation continued for some time before ceasing. Addition of the drug to exponential phase yeast cultures resulted in an approximately 45% increase in cell number before cell division ceased and yeast accumulated in both budded and unbudded stages of the cell cycle. Accumulation of trichloroacetic acid precipitable radiolabelled protein and nucleic acid continued unchanged for some time following addition of tunicamycin; however, after a while a reduced rate of accumulation was noted.
衣霉素是一种抗菌剂，它抑制导致蛋白质N-糖基化的多萜醇途径的第一步反应。衣霉素对二态真菌白色念珠菌生长的影响因该生物体的生长阶段而异。将衣霉素添加到静止期酵母细胞中会抑制这些细胞在任何一种形态下恢复生长，因为培养物无法启动酵母芽或芽管形成。当将衣霉素添加到生长中的细胞中时，生长受到抑制但并非立即受到抑制。当将其添加到芽管培养物中时，核分裂和隔膜形成会持续一段时间才停止。将该药物添加到指数期酵母培养物中会导致在细胞分裂停止且酵母在细胞周期的出芽和未出芽阶段积累之前，细胞数量增加约45%。添加衣霉素后，三氯乙酸可沉淀的放射性标记蛋白质和核酸的积累在一段时间内持续不变；然而，过了一段时间后，积累速率降低。
