Gonzalez K, Udovichenko I, Cunnick J, Takemoto D J
Department of Biology, Kansas State University, Manhattan 66506.
Curr Eye Res. 1993 Apr;12(4):373-7. doi: 10.3109/02713689308999462.
Using isozyme-specific anti-peptide antisera against peptides from the alpha-, beta-, gamma-, delta-, epsilon-, and zeta-isoforms of brain protein kinase C (PKC), we have identified proteins in bovine lens epithelial cells, in culture, that were reactive with these antisera. Western blots of lens epithelial cell homogenates showed that PKC-alpha antisera reacted with a major protein, and PKC-gamma antisera reacted with a minor protein. When the lens epithelial cells were cultured in media supplemented with 40 mM galactose, to model the conditions of sugar cataracts, a decrease in PKC-gamma, but not in PKC-alpha was observed. These were normalized if the cells were cultured in 40 mM galactose media supplemented with an inhibitor of aldose reductase, Tolrestat (10 microM). These results suggest that changes in PKC isoforms occur in the galactosemic diabetic state.
利用针对脑蛋白激酶C（PKC）的α、β、γ、δ、ε和ζ同工型的肽段的同工酶特异性抗肽抗血清，我们在培养的牛晶状体上皮细胞中鉴定出了与这些抗血清反应的蛋白质。晶状体上皮细胞匀浆的蛋白质免疫印迹显示，PKC-α抗血清与一种主要蛋白质反应，PKC-γ抗血清与一种次要蛋白质反应。当晶状体上皮细胞在补充有40 mM半乳糖的培养基中培养以模拟糖性白内障的条件时，观察到PKC-γ减少，但PKC-α没有减少。如果细胞在补充有醛糖还原酶抑制剂托瑞司他（10 μM）的40 mM半乳糖培养基中培养，这些情况则会恢复正常。这些结果表明，在半乳糖血症性糖尿病状态下PKC同工型会发生变化。
