Gonzalez-Charneco K, Takemoto D
Division of Biology, Kansas State University, Manhattan 66506, USA.
Histochem Cell Biol. 1998 Jul;110(1):89-94. doi: 10.1007/s004180050269.
The localization of the two major isoforms of protein kinase C (PKC), PKCalpha and PKCgamma, present in normal and galactosemic bovine lens epithelial cells in culture, was determined using PKC isoform-specific antisera and visualized with FITC-conjugated secondary antisera. The results indicated that the localization of PKC changed upon exposure to 40 mM galactose after 1 day. The subcellular distribution of control cells was cytoplasmic and perinuclear for PKCalpha, while, in 40 mM galactose-treated cells, PKCalpha was also localized to nuclei. In contrast, upon exposure to 40 mM galactose the PKCgamma of the lens epithelial cells was observed in nucleoli. These results suggest that the subcellular distribution of the PKC isoforms in bovine lens epithelial cells differs and is altered upon exposure to 40 mM galactose.
利用蛋白激酶C（PKC）亚型特异性抗血清，测定了培养的正常和半乳糖血症牛晶状体上皮细胞中两种主要PKC亚型PKCα和PKCγ的定位，并通过异硫氰酸荧光素（FITC）偶联的二抗进行可视化观察。结果表明，1天后暴露于40 mM半乳糖时，PKC的定位发生了变化。对照细胞中PKCα的亚细胞分布为细胞质和核周，而在40 mM半乳糖处理的细胞中，PKCα也定位于细胞核。相反，暴露于40 mM半乳糖后，在核仁中观察到晶状体上皮细胞的PKCγ。这些结果表明，牛晶状体上皮细胞中PKC亚型的亚细胞分布不同，且在暴露于40 mM半乳糖后会发生改变。
