Kochanowski B, Leopold K, Fischer W
Institut für Biochemie der Medizinischen Fakultät, Universität Erlangen-Nürnberg, Germany.
Eur J Biochem. 1993 Jun 15;214(3):757-61. doi: 10.1111/j.1432-1033.1993.tb17977.x.
The lipoteichoic acid of Streptococcus sanguis DSM 20567 contains a poly(glycerophosphate) chain, with 49% of the glycerophosphate residues being substituted with D-alanine ester, 35% with alpha-D-glucopyranosyl and alpha-isomalto-oligosaccharide residues. Analysis of molecular species by affinity chromatography on concanavalin A showed all chains to be substituted and alanine ester and glycosyl residues to be present on the same rather than on separate chains. Molecular species varied in the length of the poly(glycerophosphate) chain, the extent of glycosylation, and had a constant alanine-ester content. An alkali-hydrolysis procedure revealed a distribution pattern between random and regular for the glycosyl substituents and suggested a similar distribution for the alanyl residues which occupy the free positions between the glycosyl substituents.
血链球菌DSM 20567的脂磷壁酸含有一条聚（甘油磷酸）链，其中49%的甘油磷酸残基被D-丙氨酸酯取代，35%被α-D-吡喃葡萄糖基和α-异麦芽糖寡糖残基取代。通过伴刀豆球蛋白A亲和色谱分析分子种类表明，所有链均被取代，丙氨酸酯和糖基残基存在于同一条链上，而非不同链上。分子种类在聚（甘油磷酸）链的长度、糖基化程度方面存在差异，且丙氨酸酯含量恒定。碱水解程序揭示了糖基取代基在随机分布和规则分布之间的分布模式，并表明占据糖基取代基之间游离位置的丙氨酰残基也有类似分布。
