Hirano T, Takasaki S, Hedrick J L, Wardrip N J, Amano J, Kobata A
Department of Biochemistry, University of Tokyo, Japan.
Eur J Biochem. 1993 Jun 15;214(3):763-9. doi: 10.1111/j.1432-1033.1993.tb17978.x.
The O-linked sugar chains were liberated from porcine zona pellucida glycoproteins by alkaline-borohydride treatment and the structures of neutral oligosaccharides were determined. The major oligosaccharides had the following structures with type-1 cores, (Gal beta 1-->4GlcNAc beta 1-->3)1 3-Gal bdta 1-->3N-acetylgalactosaminitol (54%) and GlcNAc beta 1-->3(Gal beta 1-->4GlcNAc beta 1-->3)0-1Gal beta 1-->3N- acetylgalactosaminitol (16%). Approximately 6% of the oligosaccharides had the structure with a type-3 core, Gal beta 1-->4GlcNAc beta 1-->3N-acetylgalactosaminitol. Oligosaccharides having alpha-galactose (2%) and beta-GalNAc (4%) at their non-reducing termini were also found as minor components.
通过碱硼氢化物处理从猪透明带糖蛋白中释放出 O 连接糖链,并确定了中性寡糖的结构。主要寡糖具有以下 1 型核心结构:(Galβ1→4GlcNAcβ1→3)1-3-Galβ1→3N-乙酰半乳糖胺醇(54%)和 GlcNAcβ1→3(Galβ1→4GlcNAcβ1→3)0-1Galβ1→3N-乙酰半乳糖胺醇(16%)。约 6%的寡糖具有 3 型核心结构,即 Galβ1→4GlcNAcβ1→3N-乙酰半乳糖胺醇。在非还原末端具有α-半乳糖(2%)和β-GalNAc(4%)的寡糖也作为次要成分被发现。