Hokke C H, Damm J B, Kamerling J P, Vliegenthart J F
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
FEBS Lett. 1993 Aug 23;329(1-2):29-34. doi: 10.1016/0014-5793(93)80186-x.
Structural analysis by 1D and 2D 1H NMR spectroscopy of three acidic O-linked oligosaccharide alditols, released from porcine zona pellucida glycoproteins by alkaline borohydride treatment, afforded the following structures: Gal beta 1-4(6SO4)GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-3GalNAc- ol Neu5Gc alpha 2-3Gal beta 1-4(6SO4-)GlcNAc beta 1-3Gal beta 1- 4GlcNAc beta 1-3Gal beta 1-3GalNAc-ol Neu5Ac alpha 2-3Gal beta 1-4(6SO4-)GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1- 3Gal beta 1-3GalNAc-ol These oligosaccharides are the smallest compounds that contain the structural elements which are present in the acidic, high-molecular mass O-linked carbohydrate chains of porcine zona pellucida glycoproteins.