Koh C Y, Phillips J T
Graduate Program in Immunology, University of Texas Southwestern Medical Center, Dallas 75235-9036.
Mol Cell Endocrinol. 1993 Apr;92(2):R21-5. doi: 10.1016/0303-7207(93)90021-b.
Prolactin receptor (PRLr) expression and distribution in thymus, spleen, bone marrow, lymph nodes, and peripheral blood lymphocytes from young adult Lewis rats are analyzed using single-color flow cytometry and a well-characterized monoclonal antibody directed against the rat liver PRLr. The in vivo effects of regional immunization on PRLr expression are also examined. PRLr is found to be widely distributed among cells of the immune system and demonstrates lymphoid tissue-specific patterns of expression. Footpad immunization caused the rapid, but transient, induction of PRLr expression in the draining lymph node, with only modest effects on PRLr expression in other distant lymphoid tissues. These studies indicate that PRL may be capable of direct interaction with the immune system through differential expression of the PRL cell surface receptor on select lymphoid target cell populations.
使用单色流式细胞术和一种针对大鼠肝脏催乳素受体（PRLr）的特性明确的单克隆抗体，分析成年Lewis大鼠胸腺、脾脏、骨髓、淋巴结和外周血淋巴细胞中PRLr的表达和分布。还研究了局部免疫对PRLr表达的体内影响。发现PRLr广泛分布于免疫系统细胞中，并表现出淋巴组织特异性表达模式。足垫免疫导致引流淋巴结中PRLr表达迅速但短暂的诱导，对其他远处淋巴组织中PRLr表达的影响较小。这些研究表明，PRL可能能够通过在特定淋巴靶细胞群体上差异表达PRL细胞表面受体与免疫系统直接相互作用。
