Neidhart M
Department of Rheumatology, University Hospital Zurich, Switzerland.
Experientia. 1996 Sep 15;52(9):900-8. doi: 10.1007/BF01938878.
The aim of this study was to investigate the effects of Freund's complete adjuvant (FCA) on the diurnal rhythms of hormonal parameters in serum and ornithine decarboxylase (ODC) activity in various tissues of male rats. On days 1-2 after FCA, increase of ODC activity (used to evaluate the level of activation) was observed in the hypothalamus, pituitary gland, adrenal medulla, adrenal cortex, liver and lymphoid tissues, while the ODC activity in the kidney was reduced. This was accompanied by an increase in serum corticosterone. On days 3-4 after FCA, ODC activity remained elevated in the pituitary gland, liver and lymphoid tissues, while the ODC activity in the testes and pancreas was reduced; kidney ODC activity returned to baseline. This was associated with increased serum levels of prolactin (Prl) and luteinizing hormone, but decreased growth hormone, testosterone and insulin. The increase in ODC activity in the thymus, as well as the reduced ODC activity in the testes and kidney, can be obtained with paraffin. Furthermore, bromocryptine microcapsules (CBLA) reduced the FCA-induced increase of ODC activity in the pituitary gland, liver and lymphoid tissues (days 3-4) but did not affect the changes in other tissues. The increase in ODC activity in the pituitary gland, liver and lymphoid tissues is specific for FCA. A role for Prl in the induction of ODC in liver and lymphoid tissues is suggested by the fact that CBLA suppresses this enhancement.
本研究的目的是探讨弗氏完全佐剂（FCA）对雄性大鼠血清激素参数昼夜节律以及各组织中鸟氨酸脱羧酶（ODC）活性的影响。在给予FCA后的第1 - 2天，下丘脑、垂体、肾上腺髓质、肾上腺皮质、肝脏和淋巴组织中ODC活性（用于评估激活水平）升高，而肾脏中的ODC活性降低。同时血清皮质酮增加。在给予FCA后的第3 - 4天，垂体、肝脏和淋巴组织中的ODC活性仍保持升高，而睾丸和胰腺中的ODC活性降低；肾脏ODC活性恢复到基线水平。这与血清催乳素（Prl）和黄体生成素水平升高，但生长激素、睾酮和胰岛素水平降低有关。胸腺中ODC活性的增加以及睾丸和肾脏中ODC活性的降低可以通过石蜡获得。此外，溴隐亭微囊（CBLA）减少了FCA诱导的垂体、肝脏和淋巴组织中ODC活性的增加（第3 - 4天），但不影响其他组织的变化。垂体、肝脏和淋巴组织中ODC活性的增加是FCA特有的。CBLA抑制这种增强作用这一事实表明Prl在肝脏和淋巴组织中ODC诱导中起作用。
