Ohara R, Miyoshi J, Aoki M, Toyoshima K
Department of Oncogene Research, Osaka University.
Jpn J Cancer Res. 1993 May;84(5):518-25. doi: 10.1111/j.1349-7006.1993.tb00170.x.
We cloned and analyzed the murine cot proto-oncogene and examined its tissue-specific expression in fetal, newborn and adult mice. Genomic cot DNA consists of eight exons, spanning more than 25 kb, and all intron-exon borders are well conserved as compared to the human homolog. Analysis of the full-length cot cDNA revealed that it contained an open reading frame of 1,401 nucleotides, like human cot proto-oncogene. The sequence identity between murine and human cot gene is 84.4% at the nucleotide level and 93.9% at the deduced amino acid level. On northern blot analysis of poly (A)+ RNA, the cot message was detected at 2.9 kb in size. Expression of the cot gene was observed in many tissues from fetal to adult mice, though the level of expression was low in all tissues examined.
我们克隆并分析了小鼠的原癌基因cot，并检测了其在胎儿、新生和成年小鼠中的组织特异性表达。基因组cot DNA由八个外显子组成，跨度超过25 kb，与人类同源基因相比，所有内含子-外显子边界都高度保守。对全长cot cDNA的分析表明，它包含一个1401个核苷酸的开放阅读框，与人类原癌基因cot相似。小鼠和人类cot基因在核苷酸水平上的序列同一性为84.4%，在推导的氨基酸水平上为93.9%。在聚腺苷酸加尾RNA的Northern印迹分析中，检测到大小为2.9 kb的cot信息。在从胎儿到成年小鼠的许多组织中都观察到了cot基因的表达，尽管在所检测的所有组织中表达水平都很低。
