San Francisco M J, Keenan R W
Department of Biological Sciences, Texas Tech University, Lubbock 79409.
J Bacteriol. 1993 Jul;175(13):4263-5. doi: 10.1128/jb.175.13.4263-4265.1993.
Uptake of [14C]galacturonic acid in Erwinia chrysanthemi was found to be stimulated during growth on pectin and its degradation products, saturated digalacturonic acid and galacturonic acid. Cells isolated from macerated potato tissue also showed increased levels of uptake activity for this molecule compared with those showed by glycerol-grown cells. Uptake was found to be an active process, and it displayed saturation kinetics. An Escherichia coli galacturonic acid transport mutant harboring the E. chrysanthemi exuT gene(s) for galacturonic acid uptake was able to transport galacturonic acid but unable to take up the dimer [3H]digalacturonic acid.
研究发现，在欧文氏菌菊苣变种于果胶及其降解产物（饱和二半乳糖醛酸和半乳糖醛酸）上生长期间，[14C]半乳糖醛酸的摄取受到刺激。与甘油培养的细胞相比，从浸软的马铃薯组织中分离出的细胞对该分子的摄取活性水平也有所提高。摄取是一个主动过程，并且呈现出饱和动力学。携带欧文氏菌菊苣变种半乳糖醛酸摄取exuT基因的大肠杆菌半乳糖醛酸转运突变体能够转运半乳糖醛酸，但无法摄取二聚体[3H]二半乳糖醛酸。
