Van Gijsegem F, Toussaint A
J Bacteriol. 1983 Jun;154(3):1227-35. doi: 10.1128/jb.154.3.1227-1235.1983.
Using the RP4::mini-Mu pULB113 plasmid, an RP4 derivative carrying a deleted Mu prophage which allows the plasmid to pick up any chromosomal DNA segment to form R' plasmids, we cloned all of the genes of Erwinia carotovora involved in the catabolism of the hexuronates and in the transport of these substrates. With the R' plasmids we isolated, we performed complementation analysis and found that, in the Erwinia carotovora strain we used, the genes involved in the catabolism of the hexuronates are clustered in four regions of the chromosome. This genetic organization is compared with that of Escherichia coli K-12.
我们使用RP4::mini-Mu pULB113质粒（一种携带缺失Mu原噬菌体的RP4衍生物，该原噬菌体缺失使得质粒能够获取任何染色体DNA片段以形成R'质粒），克隆了胡萝卜软腐欧文氏菌中所有参与己糖醛酸分解代谢及这些底物转运的基因。利用我们分离得到的R'质粒，我们进行了互补分析，结果发现在我们所使用的胡萝卜软腐欧文氏菌菌株中，参与己糖醛酸分解代谢的基因聚集在染色体的四个区域。将这种基因组织与大肠杆菌K-12的进行了比较。
