胃液中尿素和铵浓度的测量。
Measurement of urea and ammonium concentrations in gastric juice.
作者信息
Neithercut W D, el Nujumi A M, McColl K E
机构信息
Department of Chemical Pathology, Wirral Hospital NHS Trust, Merseyside.
出版信息
J Clin Pathol. 1993 May;46(5):462-4. doi: 10.1136/jcp.46.5.462.
AIM
To study the effect of known interference in the measurement of urea and ammonium concentrations in samples of gastric juice.
METHODS
The effect of pH and ammonium concentration on the o-pthalaldehyde method, the diacetylmonoxime method, a Berthelot linked method and an enzymatic urease method for the measurement of urea in gastric juice was therefore conducted. An enzymatic method of the measurement of ammonium in gastric juice was also assessed.
RESULTS
The o-pthalaldehyde and the enzymatic urease methods were unaffected by a low gastric juice pH, ammonium concentrations of 10 mmol/l, and had interassay coefficients of variation of 3.9-5.6% and 2.8-10.6%, respectively, over a urea concentration of 2.5 mmol/l-20 mmol/l. The Berthelot linked method resulted in low gastric juice urea concentrations. The enzymatic method of ammonium measurement also proved suitable when the effect of low gastric juice pH was controlled.
CONCLUSION
Interference by low pH did not explain the differences in reports of gastric juice urea or ammonium concentrations.
目的
研究已知干扰因素对胃液样本中尿素和铵浓度测量的影响。
方法
因此，研究了pH值和铵浓度对用于测量胃液中尿素的邻苯二甲醛法、二乙酰一肟法、贝托洛偶联法和酶促脲酶法的影响。还评估了一种测量胃液中铵的酶法。
结果
邻苯二甲醛法和酶促脲酶法不受低胃液pH值、10 mmol/l铵浓度的影响，在2.5 mmol/l至20 mmol/l的尿素浓度范围内，批内变异系数分别为3.9%至5.6%和2.8%至10.6%。贝托洛偶联法导致胃液尿素浓度较低。当控制低胃液pH值的影响时，铵测量的酶法也被证明是合适的。
结论
低pH值的干扰并不能解释胃液尿素或铵浓度报告中的差异。
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