Bergmanson J P, Ross R N
University of Houston, College of Optometry, TX 77004.
J Am Optom Assoc. 1993 May;64(5):308-14.
The purpose of this study was to challenge the cornea in vivo with a lens care solution (ReNu Multi-Purpose Solution, Bausch & Lomb) formulated with polyaminopropyl biguanide at a higher than standard concentration of 1.0 ppm (1.0 x 10(-6) percent) rather than the marketed 0.5 ppm (0.5 x 10(-6) percent) and to evaluate any cytotoxic epithelial response. Test solutions included the modified formulation of Bausch & Lomb ReNu Multi-Purpose Solution (poloxamine, EDTA, borate buffer, and polyaminopropyl biguanide) and Bausch & Lomb Hypo-Clear unpreserved, isotonic saline. A dose of 50 microL of each solution was administered to the superior limbus of 10 pigmented rabbits free of anterior segment disease. Neither the unpreserved saline solution nor the more highly concentrated multipurpose solution produced significant toxic effects on the corneal epithelial cells and compared favorably with previously reported incidences of desquamating cells in the normal corneal epithelium.
本研究的目的是，使用一种含有高于标准浓度1.0 ppm（1.0×10⁻⁶%）而非市售的0.5 ppm（0.5×10⁻⁶%）的聚氨基丙基双胍配制的隐形眼镜护理液（博士伦多功能护理液）对角膜进行体内挑战，并评估任何细胞毒性上皮反应。测试溶液包括博士伦多功能护理液的改良配方（泊洛沙明、乙二胺四乙酸、硼酸盐缓冲液和聚氨基丙基双胍）以及博士伦无防腐剂等渗生理盐水。将50微升每种溶液滴入10只无前房疾病的有色家兔的上睑缘。未保存的盐溶液和浓度更高的多功能溶液均未对角膜上皮细胞产生显著毒性作用，且与先前报道的正常角膜上皮中脱屑细胞的发生率相比表现良好。
