Mikkola T, Ristimäki A, Viinikka L, Ylikorkala O
Department of Obstetrics and Gynecology, Helsinki University Central Hospital, University of Helsinki, Finland.
Life Sci. 1993;53(3):283-9. doi: 10.1016/0024-3205(93)90680-2.
Prostacyclin (PGI2), a powerful vasodilatory prostanoid, and endothelin-1 (ET-1), a potent vasoconstrictive peptide, are produced by vascular endothelial cells. We show that human serum (10%) caused a 3.2-fold stimulation both in PGI2 and ET-1 synthesis in human endothelial cells cultured from umbilical veins, and human plasma (10%) stimulated productions of both 1.6- and 1.7-fold, respectively. In addition, releasates from thrombin-activated platelets (20 x 10(9) platelets/l) caused a 1.9-fold increase in PGI2 and a 1.4-fold increase in the ET-1 synthesis. Releasates from frozen-thawed and sonicated platelets (20 x 10(9) platelets/l) caused a 3.6-fold increase in PGI2 release but did not affect ET-1 production. We thus conclude that, in normal situation, endothelial stimulating activity present in plasma perhaps plays a role in the regulation of endothelial function, whereas platelet-derived activity in serum may be important at site of thrombosis.
前列环素（PGI2）是一种强效血管舒张性前列腺素，内皮素-1（ET-1）是一种强力血管收缩性肽，二者均由血管内皮细胞产生。我们发现，人血清（10%）使脐静脉培养的人内皮细胞中PGI2和ET-1的合成均增加了3.2倍，人血浆（10%）则分别使二者的产生量增加了1.6倍和1.7倍。此外，凝血酶激活的血小板（20×10⁹个血小板/升）的释放物使PGI2增加了1.9倍，ET-1的合成增加了1.4倍。冻融并超声处理的血小板（20×10⁹个血小板/升）的释放物使PGI2释放增加了3.6倍，但不影响ET-1的产生。因此我们得出结论，在正常情况下，血浆中存在的内皮刺激活性可能在调节内皮功能中发挥作用，而血清中血小板衍生的活性在血栓形成部位可能很重要。
