Ristimäki A, Renkonen R, Saijonmaa O, Ylikorkala O, Viinikka L
Children's Hospital, University of Helsinki, Finland.
Life Sci. 1991;49(8):603-9. doi: 10.1016/0024-3205(91)90259-e.
Human serum stimulated the synthesis of a vasoconstrictive peptide, endothelin-1 (ET-1), and a vasodilatory prostanoid, prostacyclin (PGI2), by cultured human umbilical vein endothelial cells in a concentration- and time-dependent manner. Incubation in 20% concentration of the serum for 24 h stimulated ET-1 synthesis almost six-fold while PGI2 production increased two-fold. In addition, a tumor-promoting phorbol ester, phorbol 12-myristate 13-acetate (PMA), inhibited the serum-induced ET-1 production and stimulated PGI2 synthesis in a concentration- and time-dependent manner. Our results suggest that human serum derived factor(s) stimulate the production of vasoconstrictive ET-1 more potently than the synthesis of vasodilatory PGI2 by human vascular endothelial cells and that the production of these agents is differentially regulated by PMA.
人血清能以浓度和时间依赖性方式刺激培养的人脐静脉内皮细胞合成血管收缩肽内皮素 -1（ET -1）和血管舒张类前列腺素前列环素（PGI2）。在20%浓度的血清中孵育24小时，刺激ET -1合成增加近6倍，而PGI2生成增加2倍。此外，一种促肿瘤佛波酯，佛波醇12 -肉豆蔻酸酯13 -乙酸酯（PMA），以浓度和时间依赖性方式抑制血清诱导的ET -1生成并刺激PGI2合成。我们的结果表明，人血清衍生因子刺激人血管内皮细胞产生血管收缩性ET -1的作用比产生血管舒张性PGI2的作用更强，并且这些介质的产生受到PMA的差异调节。
