Radunović A, Bradbury M W, Delves H T
Biomedical Sciences Division, King's College London, Strand, UK.
Analyst. 1993 May;118(5):533-6. doi: 10.1039/an9931800533.
Atomic absorption spectrometry with electrothermal atomization was used for the determination of aluminium in brain, liver, spleen, kidney cortex, skeletal muscle and bone of the rat following digestion by nitric acid and in serum following simple dilution and in situ oxygen ashing. The method of standard additions in the presence of a chemical modifier, ammonium dihydrogen-phosphate, was essential for bone tissues. The detection limits ranged from 3 to 58 ng per gram of wet mass of tissue and were 4-19 times lower than the observed physiological levels of aluminium. The between-day precision for serum was 8.9% at a mean concentration of 6.8 micrograms I-1 and 2.4% at a mean concentration of 125.3 micrograms I-1. Additionally, repeated analyses of National Institute of Standards and Technology Standard Reference Material 1577b Bovine Liver gave a relative standard deviation of 12.2% (mean concentration = 0.8 microgram g-1). Of the tissues studied, bone had at least ten times higher levels of aluminium than others (0.959 +/- 0.322 micrograms g-1). The aluminium concentration in cerebellum (0.073 +/- 0.043 micrograms g-1) was approximately twice that in the cerebral hemisphere (0.034 +/- 0.009 micrograms g-1).
采用电热原子化原子吸收光谱法测定大鼠经硝酸消化后的脑、肝、脾、肾皮质、骨骼肌和骨骼中的铝含量，以及简单稀释和原位氧灰化后的血清中的铝含量。对于骨组织，在化学改性剂磷酸二氢铵存在下的标准加入法至关重要。检测限为每克湿组织3至58纳克，比观察到的铝的生理水平低4至19倍。血清的日间精密度在平均浓度为6.8微克/升时为8.9%，在平均浓度为125.3微克/升时为2.4%。此外，对国家标准与技术研究院标准参考物质1577b牛肝进行的重复分析得出相对标准偏差为12.2%（平均浓度 = 0.8微克/克）。在所研究的组织中，骨中的铝含量至少比其他组织高十倍（0.959±0.322微克/克）。小脑（0.073±0.043微克/克）中的铝浓度约为大脑半球（0.034±0.009微克/克）中的两倍。
