Leung F Y, Edmond P
Department of Clinical Biochemistry, London Health Sciences Centre, Ontario, Canada.
Clin Biochem. 1997 Jul;30(5):399-403. doi: 10.1016/s0009-9120(97)00043-x.
To employ an electrothermal atomic absorption spectrometry (ETAAS) method with chemical modifiers for the determination of silicon (Si) in serum and tissues.
Si was measured in serum of chronic hemodialysis patients, and in fibrous breast capsule tissues following silicone-gel implant removal. Tissue was dried, and digested with concentrated nitric acid prior to analysis. A chemical modifier, which included lanthanum oxide and ammonium phosphate, was used to dilute the serum, and digested tissue samples (1:4) before atomization. Si was determined at 251.6 nm in a graphite fumace using an atomic absorption spectrophotometer.
The method was linear (to 1000 micrograms/L), and precise (CV 5.9% at 281 micrograms/L and 8.4% at 73 micrograms/L). Recovery of Si in spiked serum and breast tissue samples was between 97-104%. Reference values for women on a normal diet and no exposure to silicone implants gave serum Si to 30 to 209 micrograms/L (n = 60), and breast tissue levels of 0.25 to 2.4 micrograms/g dry wt (n = 48). Si in breast capsule from women exposed to silicone breast implants varied from 29 to 496 micrograms/g dry wt (n = 10). Serum Si in hemodialysis patients (n = 53) ranged from 900 to 3300 micrograms/L.
We conclude that our chemically modified ETAAS method is suitable for Si determination in normal and elevated human serum and tissue specimens.
采用带化学改进剂的电热原子吸收光谱法（ETAAS）测定血清和组织中的硅（Si）。
测定慢性血液透析患者血清中的硅，以及硅胶植入物取出后乳腺纤维包膜组织中的硅。组织经干燥处理，分析前用浓硝酸消化。使用包含氧化镧和磷酸铵的化学改进剂将血清和消化后的组织样品按1:4稀释后进行雾化。使用原子吸收分光光度计在石墨炉中于251.6nm处测定硅。
该方法呈线性（至1000微克/升），且精密度良好（281微克/升时变异系数为5.9%，73微克/升时为8.4%）。加标血清和乳腺组织样品中硅的回收率在97%至104%之间。正常饮食且未接触硅胶植入物的女性的参考值为血清硅30至209微克/升（n = 60），乳腺组织水平为0.25至2.4微克/克干重（n = 48）。接触硅胶乳房植入物的女性乳腺包膜中的硅含量在29至496微克/克干重之间（n = 10）。血液透析患者（n = 53）的血清硅含量在900至3300微克/升之间。
我们得出结论，我们的化学改进ETAAS方法适用于测定正常及升高水平的人体血清和组织样本中的硅。
