Papasouliotis K, Gruffydd-Jones T J, Sparkes A H, Cripps P J, Millard W G
Department of Veterinary Medicine, University of Bristol, England.
Am J Vet Res. 1993 Jun;54(6):840-4.
Intestinal permeability was assessed in 12 healthy cats by use of a differential sugar absorption test. A 50-ml isotonic aqueous solution containing a combination of 1.8 g of the disaccharide lactulose and 1.7 g of the monosaccharide mannitol was administered to cats via nasogastric tube. Urine was collected after 6 hours, and all urine samples were analyzed the same day, using a gas-liquid chromatographic technique (GLC) and an enzymatic assay (ENZ). Median urinary recovery of lactulose was 0.27 and 0.54% determined by GLC and ENZ, respectively. Differences between these groups were statistically significant (P = 0.023), and correlation between assays was high (r = 0.94, P < 0.01). Median urinary recovery of mannitol was 1.93 and 2.09% for GLC and ENZ, respectively. There were no statistically significant differences between these groups and the correlation between assays was high (r = 0.85, P < 0.01). The median lactulose-to-mannitol ratio was 0.29, using GLC, and was 0.52, using ENZ. Correlation of these ratios was again high (r = 0.93, P < 0.01).
通过使用差异糖吸收试验评估了12只健康猫的肠道通透性。将含有1.8克双糖乳果糖和1.7克单糖甘露醇的50毫升等渗水溶液通过鼻胃管给予猫。6小时后收集尿液，所有尿液样本在同一天使用气液色谱技术（GLC）和酶法测定（ENZ）进行分析。通过GLC和ENZ测定的乳果糖尿回收率中位数分别为0.27%和0.54%。这些组之间的差异具有统计学意义（P = 0.023），并且测定之间的相关性很高（r = 0.94，P < 0.01）。GLC和ENZ测定的甘露醇尿回收率中位数分别为1.93%和2.09%。这些组之间没有统计学上的显著差异，并且测定之间的相关性很高（r = 0.85，P < 0.01）。使用GLC时，乳果糖与甘露醇的中位数比率为0.29，使用ENZ时为0.52。这些比率的相关性同样很高（r = 0.93，P < 0.01）。
