Takumida M, Harada Y
Department of Otolaryngology, Hiroshima University School of Medicine, Japan.
Auris Nasus Larynx. 1993;20(1):1-9. doi: 10.1016/s0385-8146(12)80205-7.
The ultrastructure of ciliary interconnecting systems as well as changes after incubation in frog Ringer's solution of the bullfrog vestibular end organs were investigated by using a scanning electron microscopy. The plasma membrane of the sensory hairs showed rather rough appearance. Side links emerged from the plasma membrane and tightly connected neighboring cilia. The tip links stretched from the tips of the stereocilia to their taller neighbors. These tip links were arranged in a same direction towards the kinocilium. These findings indicate that the side links may keep the cilia arranged in a bundle and the tip links may be involved in sensory cell transduction system. Changes in the surface texture and ciliary interconnecting systems were detected 2 hrs after incubation in frog Ringer's solution. Such changes included an increase in granularity of the surface membrane, fracturing and disappearance of all types of ciliary interconnections, especially side links. The tip links were more durable than side links. This indicates that the physiological studies of bullfrog hair bundle are justified across a 1-hr interval from the time the temporal bones are removed from the animal.
运用扫描电子显微镜研究了牛蛙前庭终器纤毛连接系统的超微结构以及在蛙林格氏液中孵育后的变化。感觉毛的质膜呈现出相当粗糙的外观。侧链从质膜伸出并紧密连接相邻的纤毛。顶链从静纤毛的尖端延伸至其较高的相邻纤毛。这些顶链朝着动纤毛以相同方向排列。这些发现表明，侧链可能使纤毛保持成束排列，而顶链可能参与感觉细胞转导系统。在蛙林格氏液中孵育2小时后，检测到表面纹理和纤毛连接系统的变化。此类变化包括表面膜颗粒度增加、所有类型的纤毛连接断裂和消失，尤其是侧链。顶链比侧链更持久。这表明从动物身上取出颞骨后的1小时内对牛蛙毛束进行生理学研究是合理的。
