Zaphiropoulos P G, Wood T
Department of Medical Nutrition, Karolinska Institute, Huddinge, Sweden.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):1006-13. doi: 10.1006/bbrc.1993.1725.
The reverse transcriptase (RT)/polymerase chain reaction (PCR) methodology was used with a set of primers that corresponds to two conserved regions of the cytochrome P450s of subfamily 2C, in order to identify the members of this group of enzymes that are expressed at the RNA level in rat brain. Seven RT/PCR clones were sequenced from female brain, and four were found to code for P450 2C7 while three for P450 2C12. Using the same methodology nine RT/PCR clones were sequenced from olfactory lobes of ethanol treated male rats and three were found to code for P450 2C6, one for P450 2C11, three for P450 2C12 and two for P450 2C23. Neither ethanol administration nor hypophysectomy or growth hormone treatment had significant effects on the expression levels of these cytochromes in the brain.
采用逆转录酶（RT）/聚合酶链反应（PCR）方法，使用一组与细胞色素P450 2C亚家族的两个保守区域相对应的引物，以鉴定在大鼠脑中RNA水平表达的这组酶的成员。从雌性大鼠脑中对7个RT/PCR克隆进行了测序，发现其中4个编码细胞色素P450 2C7，3个编码细胞色素P450 2C12。使用相同方法，对乙醇处理的雄性大鼠嗅叶中的9个RT/PCR克隆进行了测序，发现其中3个编码细胞色素P450 2C6，1个编码细胞色素P450 2C11，3个编码细胞色素P450 2C12，2个编码细胞色素P450 2C23。给予乙醇、垂体切除或生长激素处理对脑中这些细胞色素的表达水平均无显著影响。
