Geffner M E, Bailey R C, Bersch N, Vera J C, Golde D W
Department of Pediatrics, University of California, Los Angeles 90024.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):1216-23. doi: 10.1006/bbrc.1993.1755.
The cause of short stature in African Pygmies is unknown, but some evidence suggests that they are GH resistant. Since IGF-I mediates many actions of GH, we sought to determine if Pygmy tissue is responsive to IGF-I. We established HTLV-II-transformed cell lines from 1 Efe Pygmy, 1 African control, and 3 American controls, and quantified in vitro colony formation in response to IGF-I, GH, and insulin, and assessed IGF-I receptor binding. The Pygmy T-cell line showed no clonal responsiveness following stimulation with physiologic concentrations of IGF-I or any concentration of GH, but responded normally to insulin. IGF-I binding studies showed no binding to the Pygmy T-cell line with normal binding to control cells. The primary abnormality in this Pygmy T-cell line is IGF-I resistance at the receptor level with secondary GH resistance.
非洲俾格米人身材矮小的原因尚不清楚，但一些证据表明他们对生长激素（GH）具有抗性。由于胰岛素样生长因子-I（IGF-I）介导生长激素的许多作用，我们试图确定俾格米人的组织是否对IGF-I有反应。我们从一名埃菲俾格米人、一名非洲对照和三名美国对照中建立了人嗜T淋巴细胞病毒II型（HTLV-II）转化的细胞系，并对IGF-I、GH和胰岛素刺激后的体外集落形成进行了定量分析，并评估了IGF-I受体结合情况。俾格米人T细胞系在生理浓度的IGF-I或任何浓度的GH刺激后均未表现出克隆反应，但对胰岛素反应正常。IGF-I结合研究表明，俾格米人T细胞系无结合，而对照细胞结合正常。该俾格米人T细胞系的主要异常是受体水平的IGF-I抗性及继发性GH抗性。
