Sunde R A, Dyer J A, Moran T V, Evenson J K, Sugimoto M
Dept. of Food Science & Human Nutrition, College of Human Environmental Sciences, University of Missouri, Columbia 65211.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):905-11. doi: 10.1006/bbrc.1993.1711.
We have isolated and sequenced an apparent full-length cDNA clone for phospholipid hydroperoxide glutathione peroxidase (Genbank accession number L12743) from a pig blastocyst cDNA library. The sequence encodes a polypeptide of 170 amino acids, including a TGA-encoded selenocysteine at residue 46, with a calculated M(r) of 19,492 Da. Use of this clone in Northern blot analysis of Se-deficient rat liver revealed that phospholipid hydroperoxide glutathione peroxidase mRNA levels were little affected by Se deficiency, whereas classical glutathione peroxidase mRNA levels were decreased by 90% in the same samples. Lastly, liver phospholipid hydroperoxide glutathione peroxidase mRNA levels were not elevated in female rats, in contrast to classical glutathione peroxidase.
我们从猪囊胚cDNA文库中分离并测序了一个磷脂氢过氧化物谷胱甘肽过氧化物酶的明显全长cDNA克隆（基因库登录号L12743）。该序列编码一个由170个氨基酸组成的多肽，包括第46位残基处由TGA编码的硒代半胱氨酸，计算的分子量为19492道尔顿。将此克隆用于缺硒大鼠肝脏的Northern印迹分析表明，磷脂氢过氧化物谷胱甘肽过氧化物酶mRNA水平受缺硒影响较小，而在相同样本中经典谷胱甘肽过氧化物酶mRNA水平下降了90%。最后，与经典谷胱甘肽过氧化物酶不同，雌性大鼠肝脏中磷脂氢过氧化物谷胱甘肽过氧化物酶mRNA水平并未升高。
