Yoshimura S, Takekoshi S, Watanabe K, Fujii-Kuriyama Y
Cell Biology Research Laboratory, Tokai University, Kanagawa, Japan.
Biochem Biophys Res Commun. 1988 Aug 15;154(3):1024-8. doi: 10.1016/0006-291x(88)90242-2.
The cDNA for rat glutathione peroxidase mRNA was isolated from liver cDNA library in lambda gt11 by cross-hybridization using the mouse cDNA, and it's nucleotide sequence was determined. The selenocysteine which constitutes an active center of this enzyme was encoded by TGA, a nonsense codon in general, as was the cases with mouse and human glutathione peroxidase. Northern blot analysis elucidated that the mRNA for glutathione peroxidase was markedly diminished in selenium deficient rat liver as compared with that of normal rat livers. The result suggested that the de novo synthesis of the mRNA would be regulated by selenium.
利用小鼠cDNA通过交叉杂交从λgt11载体中的大鼠肝脏cDNA文库中分离出大鼠谷胱甘肽过氧化物酶mRNA的cDNA，并测定了其核苷酸序列。构成该酶活性中心的硒代半胱氨酸由通常为无义密码子的TGA编码，小鼠和人类谷胱甘肽过氧化物酶也是如此。Northern印迹分析表明，与正常大鼠肝脏相比，缺硒大鼠肝脏中谷胱甘肽过氧化物酶的mRNA明显减少。结果表明，mRNA的从头合成受硒的调控。
