Morin L G
Clin Chem. 1977 Feb;23(2 PT. 1):205-10.
I have evaluated electrophoretic, ion-exchange, kinetic, selective activation, and immunological methods of fractionating creatine kinase isoenzymes for diagnostic purposes. The last three were unsuitable. Electrophoretic methods had superior resolution and reliability, but were relatively insensitive when conventional film-drying techniques were used. With elution from the film, sensitivity was comparable to that of the ion-exchange methods. Electrophoretic methods require more skill than the other methods. Ion-exchange methods had acceptable resolution and reliability with potentially superior sensitivity; they require less skill than electrophoresis, but require about as much time. Batch operation of ion-exchange yielded superior efficiency and sensitivity, and was otherwise comparable to column operation.
我评估了用于诊断目的的肌酸激酶同工酶分级分离的电泳法、离子交换法、动力学法、选择性活化法和免疫学法。后三种方法不合适。电泳法具有更高的分辨率和可靠性，但使用传统胶片干燥技术时相对不敏感。通过从胶片上洗脱，灵敏度与离子交换法相当。电泳法比其他方法需要更多技巧。离子交换法具有可接受的分辨率和可靠性，灵敏度可能更高；它们比电泳法所需技巧少，但所需时间大致相同。离子交换的批量操作效率和灵敏度更高，在其他方面与柱操作相当。
