Klein B, Foreman J A, Jeunelot C L, Sheehan J E
Clin Chem. 1977 Mar;23(3):504-10.
We describe a practical, technically convenient DEAE-Sephadex chromatographic column and a three-fraction salt-gradient elution procedure, with which serum creatine kinase activity is rapidly and quantitatively separated into the isoenzyme components with excellent analytical recovery of the total activity applied. The homogeneity of the isoenzyme fractions was demonstrated by electrophoresis and rechromatography. Sera assessed by the Mercer fractionation [Clin. Chem. 20, 36 (1974)] and the present procedure showed excellent agreement.
我们描述了一种实用、技术上简便的二乙氨基乙基葡聚糖（DEAE - Sephadex）色谱柱及一种三组分盐梯度洗脱程序，利用该程序可将血清肌酸激酶活性快速、定量地分离为同工酶组分，且所加总活性的分析回收率极佳。通过电泳和再色谱法证明了同工酶组分的均一性。采用默瑟分级分离法[《临床化学》20, 36 (1974)]和本程序评估的血清显示出极佳的一致性。
