Lavallee D K, Norelius J T
Clin Chem. 1977 Feb;23(2 PT. 1):282-5.
An improved method for separation and fluorescent analysis of coproporphyrin and uroporphyrin in urine was presented by Sobel, Cano, and Thiers [Clin. Chem. 20, 1397 (1974)]. Accurate determination of porphyrin concentrations by this method is impeded by the fluorescent background in urine samples, which varies from one specimen to another. By use of a straightforward procedure, zinc may be incorporated into coproporphyrin and uroporphyrin. Measurement of the difference in the fluorescent emission intensity at 650 nm for the porphyrin solutions before and after zinc incorporation compensates for fluorescent background and gives a more accurate determination.
索贝尔、卡诺和蒂尔斯[《临床化学》20, 1397 (1974)]提出了一种改进的尿液中粪卟啉和尿卟啉的分离及荧光分析方法。该方法对卟啉浓度的准确测定受到尿液样本中荧光背景的阻碍，不同样本的荧光背景有所差异。通过一种简单的程序，锌可被掺入粪卟啉和尿卟啉中。测量掺入锌前后卟啉溶液在650 nm处荧光发射强度的差异，可补偿荧光背景并实现更准确的测定。
