Valcárcel M, Gómez Hens A, Rubio S, Petidier A
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Clin Chem. 1987 Oct;33(10):1826-31.
The second-derivative synchronous fluorescence spectroscopic technique is applied to the simultaneous and direct assay of coproporphyrins and uroporphyrins in human urine. This technique resolves the overlapping conventional spectra, obviating the need for pre-analysis sample separation techniques, and measurements can be made in a single scan. The amplitudes of the derivative peaks are linearly related to uroporphyrin (0.4 to 300 micrograms/L) and coproporphyrin (0.4 to 250 micrograms/L) concentrations. The detection limit for both porphyrins is 0.1 microgram/L. Analytical recoveries range between 98 and 101%. Within- and between-assay CVs are reported. Results for 24-h urine specimens correlated well with those obtained by an extraction-ion-exchange chromatography combined method. The proposed method is inexpensive and requires no sophisticated detection equipment.
二阶导数同步荧光光谱技术用于同时直接测定人尿中的粪卟啉和尿卟啉。该技术可解析重叠的常规光谱，无需进行分析前的样品分离技术，单次扫描即可完成测量。导数峰的幅度与尿卟啉（0.4至300微克/升）和粪卟啉（0.4至250微克/升）浓度呈线性关系。两种卟啉的检测限均为0.1微克/升。分析回收率在98%至101%之间。报告了批内和批间变异系数。24小时尿液标本的结果与通过萃取-离子交换色谱联合法获得的结果相关性良好。所提出的方法成本低廉，无需复杂的检测设备。
