Leahy D T, Brien T G
J Clin Pathol. 1982 Nov;35(11):1232-5. doi: 10.1136/jcp.35.11.1232.
A simple routine method for the separation and quantification of urinary coproporphyrin and uroporphyrin using anion-exchange resin columns is described. The coproporphyrin is first removed from the urine by ether extraction. The anion exchange resin column is then used to isolate the uroporphyrin from the aqueous residue. The proposed method is compared with an existing method developed by Rimington in terms of recovery and reproducibility. Results from 15 urine specimens analysed by both methods are compared. The proposed method yielded lower values for coproporphyrin and higher values for uroporphyrin than the established method, but there was a good correlation between the two methods. This and its relative simplicity make it suitable for routine use.
本文描述了一种使用阴离子交换树脂柱分离和定量尿中粪卟啉和尿卟啉的简单常规方法。首先通过乙醚萃取从尿液中去除粪卟啉。然后使用阴离子交换树脂柱从水相残余物中分离尿卟啉。将该方法与Rimington开发的现有方法在回收率和重现性方面进行了比较。比较了两种方法对15份尿液标本的分析结果。与既定方法相比，该方法得到的粪卟啉值较低，尿卟啉值较高，但两种方法之间具有良好的相关性。这一点及其相对简单性使其适用于常规使用。
