Quirós E, García F, Cabezas T, González I, Bernal M C, Maroto M C, Piedrola G
Departamento de Microbiología (Facultad de Medicina), Universidad de Granada.
Enferm Infecc Microbiol Clin. 1993 May;11(5):241-3.
To evaluate the diagnostic use of Polymerase Chain Reaction (PCR) for the detection of HIV-DNA.
We have investigated HIV-DNA in peripheral blood mononuclear cells of 150 individuals: 50 seronegative without recognized factors to acquire HIV infection, 50 seronegative that showed risk habits and 50 seropositive. For amplification we have used primers SK38/SK39 which amplify a conserved region of 114 bp from HIV's gag gen. The detection of the amplificate was performed using solution hybridization with 32P-labelled SK 19, 6% polyacrylamide gel electrophoresis and autoradiography.
We have detected HIV-DNA at every seropositive patient (100%), 6 samples from seronegative individuals that showed risk habits and 1 sample from non-risk seronegative individuals.
Polymerase chain reaction represents a diagnostic tool at HIV infection, specially for those patients where seroconversion can not be demonstrated by conventional methods.
评估聚合酶链反应（PCR）用于检测HIV-DNA的诊断价值。
我们检测了150名个体外周血单个核细胞中的HIV-DNA，其中50名血清学阴性且无已知感染HIV的危险因素，50名血清学阴性但有高危行为，50名血清学阳性。我们使用引物SK38/SK39进行扩增，该引物可扩增HIV gag基因中一段114 bp的保守区域。扩增产物的检测采用与32P标记的SK 19进行溶液杂交、6%聚丙烯酰胺凝胶电泳和放射自显影。
我们在所有血清学阳性患者（100%）中检测到了HIV-DNA，在6名有高危行为的血清学阴性个体和1名无高危行为的血清学阴性个体中也检测到了HIV-DNA。
聚合酶链反应是HIV感染的一种诊断工具，尤其适用于那些无法通过传统方法证实血清转化的患者。
