Schmidt B L, Gschnait F
Ludwig Boltzmann-Institut für dermato-venerologische Serodiagnostik Wien.
Hautarzt. 1991 Dec;42(12):754-8.
The polymerase chain reaction (PCR) allows the detection of minimal amounts of DNA segments, and thus is used for the laboratory diagnosis of HIV (human immunodeficiency virus). In the present study a solution hybridization assay using acridinium ester-labelled probes was applied for detection of amplified HIV-1 DNA segments. Amplification was achieved by 30 cycles of the polymerase chain reaction using SK38/SK39 primers specific for a constant region of the HIV-1 gag region. Amplified products were hybridized with acridinium phenyl ester-labelled probes and measured by chemiluminescence. All of 163 blood samples obtained from HIV-infected patients (CDC II-CDC IV) were reactive, while autoradiography with a 32P-labelled SK19 probe only detected 146 patients (89.5%). The combination of PCR with chemiluminescence avoids the use of radioactive material and is sensitive and quick, allowing the "PCR results" to be reported on the same day.
聚合酶链反应（PCR）能够检测出微量的DNA片段，因此被用于人类免疫缺陷病毒（HIV）的实验室诊断。在本研究中，采用吖啶酯标记探针的溶液杂交试验来检测扩增的HIV-1 DNA片段。使用针对HIV-1 gag区域恒定区的SK38/SK39引物，通过30个循环的聚合酶链反应实现扩增。扩增产物与吖啶苯基酯标记的探针杂交，并通过化学发光进行检测。从HIV感染患者（疾病控制与预防中心II级 - 疾病控制与预防中心IV级）获得的163份血液样本全部呈阳性反应，而使用32P标记的SK19探针进行放射自显影仅检测出146例患者（89.5%）。PCR与化学发光相结合避免了使用放射性物质，且灵敏快速，能够在同一天报告“PCR结果”。
