van Heel M, Frank J
Ultramicroscopy. 1981;6(2):187-94. doi: 10.1016/0304-3991(81)90059-0.
We have developed a new technique of analysis that allows automatic classification of molecule images according to subtle differences. Computer alignment and multivariate statistical methods were used to analyze electron micrographic images of horseshoe crab hemocyanin half-molecules. The molecule projections fell into four distinct classes related to four different positions of the molecule on the grid. Averages obtained for each images subset are interpreted in terms of a three-dimensional model arrangement for the four subunits forming the half-molecule.
我们开发了一种新的分析技术，可根据细微差异对分子图像进行自动分类。利用计算机对齐和多元统计方法来分析鲎血蓝蛋白半分子的电子显微图像。分子投影可分为四类，这四类与分子在网格上的四个不同位置相关。根据构成半分子的四个亚基的三维模型排列来解释每个图像子集获得的平均值。
