粟酒裂殖酵母死细胞和活细胞中的取向介电能。实验结果与理论模型的拟合
Dielectric energy of orientation in dead and living cells of Schizosaccharomyces pombe. Fitting of experimental results to a theoretical model.
作者信息
Asencor F J, Santamaría C, Iglesias F J, Domínguez A
机构信息
Departamento de Física Aplicada, Universidad del País Vasco, Bilbao, Spain.
出版信息
Biophys J. 1993 May;64(5):1626-31. doi: 10.1016/S0006-3495(93)81509-1.
Abstract
Using the experimental data obtained with killed cells of Schizosaccharomyces pombe (1), we have formulated a theoretical model that is able to predict cell orientation for microorganisms with ellipsoidal or cylindrical shapes as a function of the frequency of the electric field and of the conductivity of the external medium. In this model, comparison of the difference in potential energy for both orientations parallel-perpendicular with the thermal agitation energy allows one to interpret the intervals where these orientations occur. The model implies that the conductivity of the cytoplasm is slightly higher than that of the external medium. This assumption is easy to understand taking into account that not all the intracytoplasmic material is released to the exterior during cell death.
摘要
利用裂殖酵母死细胞获得的实验数据(1)，我们构建了一个理论模型，该模型能够根据电场频率和外部介质的电导率预测椭圆形或圆柱形微生物的细胞取向。在这个模型中，通过比较平行-垂直两种取向的势能差与热运动能量，可以解释这些取向出现的区间。该模型表明，细胞质的电导率略高于外部介质。考虑到细胞死亡时并非所有胞内物质都释放到外部，这个假设很容易理解。
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