Wirth R, Friesenegger A, Fiedler S
Lehrstuhl für Mikrobiologie, Universität München, Federal Republic of Germany.
Mol Gen Genet. 1989 Mar;216(1):175-7. doi: 10.1007/BF00332248.
We have undertaken a systematic study to test the transformation of various species of gram-negative bacteria using the electroporation method. The data obtained show very clearly that a great variety of gram-negative bacteria--15 different species belonging to 11 different genera--including freshly isolated wild-type strains can be transformed efficiently by use of the electric-field mediated transformation technique. These include species of the families Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae, photosynthetic bacteria and strains for which transformation could not be achieved, up to now, by other methods.
我们进行了一项系统研究，以测试使用电穿孔法对各种革兰氏阴性菌进行转化。所获得的数据非常清楚地表明，利用电场介导的转化技术，可以高效地转化多种革兰氏阴性菌——属于11个不同属的15个不同物种——包括新分离的野生型菌株。这些细菌包括肠杆菌科、假单胞菌科、根瘤菌科、光合细菌以及迄今为止通过其他方法无法实现转化的菌株。
