Rao P D, Job T C, Schreibman M P
Department of Zoology, Nagpur University, India.
Brain Behav Evol. 1993;42(1):24-38. doi: 10.1159/000114138.
Seven hypothalamic nuclei and several isolated perikarya that send projections to the pituitary gland were identified following administration of cobaltous lysine or horseradish peroxidase (HRP) to severed hypophysial stalks of previously hypophysectomized catfish, Clarias batrachus. Retrogradely labelled neurons were identified in the nucleus preopticus periventricularis, suprachiasmatic nucleus, paraventricular and supraoptic divisions of the magnocellular nucleus preopticus (NPO) and nucleus lateralis tuberis. A few neurons in the paraventricular subdivision of the NPO, however, remained unfilled; these may project to extrahypophysial sites. Three other nuclei contributing to the innervation of the pituitary gland include the paraventricular organ, nucleus recessus lateralis (NRL) and nucleus recessus posterioris (NRP), all of which contain cerebrospinal fluid-contacting aminergic neurons. These three neuronal aggregations were retrogradely labelled with cobaltous lysine but not with HRP. Isolated neurons displaying hypophysial connections were identified in the organon vasculosum laminae terminalis area, in the nucleus hypothalamicus ventromedialis, and in the vicinity of the NRL and NRP. Thus the present study reveals that hypothalamic projections to the pituitary gland of catfish have their origin in several peptidergic and aminergic nuclei and perikarya hitherto unreported.
向先前已切除垂体的鲶鱼（胡子鲶）离断的垂体柄注射赖氨酸钴或辣根过氧化物酶（HRP）后，鉴定出七个下丘脑核团以及几个向垂体发送投射的孤立的神经细胞体。在室周视前核、视交叉上核、视前大细胞核（NPO）的室旁和视上部分以及外侧结节核中发现了逆行标记的神经元。然而，NPO室旁亚区的一些神经元未被标记；这些神经元可能投射到垂体外的部位。另外三个对垂体神经支配有贡献的核团包括室旁器官、外侧隐窝核（NRL）和后隐窝核（NRP），它们都含有与脑脊液接触的胺能神经元。这三个神经元聚集区用赖氨酸钴逆行标记，但未用HRP标记。在终板血管器区域、下丘脑腹内侧核以及NRL和NRP附近发现了显示与垂体有联系的孤立神经元。因此，本研究表明，鲶鱼下丘脑向垂体的投射起源于几个迄今未报道的肽能和胺能核团及神经细胞体。
