Roccheri M C, Cascino D, Giudice G
Department of Cellular and Developmental Biology A. Monroy, University of Palermo, Italy.
J Submicrosc Cytol Pathol. 1993 Apr;25(2):173-9.
The synthesis of stress proteins in Paracentrotus lividus embryos has been analysed by two-dimensional electrophoresis following either heat shock or ZnSO4 administration. As already shown for the 70-KDa hsp (heat shock proteins) a non responsive period, that is before hatching, exists followed by a responsive period in which up to 16 hsps are synthesized after heat shock, 7 of which are also synthesized after ZnSO4 treatment. Some of them pre-existed the stress, but their synthesis increases following the stress. Only few of them accumulate in amounts detectable by silver staining. The synthesis of all these proteins depends upon RNA synthesis.
通过二维电泳分析了紫球海胆胚胎在热休克或给予硫酸锌后应激蛋白的合成情况。如已显示的70-KDa热休克蛋白那样，在孵化前存在一个无反应期，随后是一个反应期，热休克后可合成多达16种热休克蛋白，其中7种在硫酸锌处理后也会合成。其中一些在应激之前就已存在，但应激后其合成增加。只有少数能通过银染检测到其积累量。所有这些蛋白质的合成均依赖于RNA合成。
