West J L
Cornell Vet. 1977 Jan;67(1):139-46.
To produce hematopoietic chimeras, 3 young, healthy calves were exposed to whole-body irradiation and, 6 days after irradiation, were injected with a bone-marrow suspension prepared from an unrelated, normal donor calf. Antibiotics were administered daily to control bacterial infection. One calf died 8 days postirradiation (PI), another died 10 days PI, and the third calf was killed in extremis 11 days PI. In addition to lesions of acute radiation injury, one to several spherical, firm, dry, reddish-black nodules were found in the liver of each calf. Hepatic changes and blood-stained urine were suggestive of bacillary hemoglobinuria. Fungi, identified by their morphologic features as members of the class Phycomycetes, were widely distributed in the tissues and lesions.
为制备造血嵌合体，对3头健康的幼年小牛进行全身照射，并在照射后6天注射由一头无关的正常供体小牛制备的骨髓悬液。每天给予抗生素以控制细菌感染。一头小牛在照射后8天死亡，另一头在照射后10天死亡，第三头小牛在照射后11天极度虚弱时被处死。除急性辐射损伤的病变外，每头小牛的肝脏中均发现1至数个球形、坚实、干燥、红黑色的结节。肝脏变化和血尿提示为细菌性血红蛋白尿。通过形态学特征鉴定为藻状菌纲成员的真菌广泛分布于组织和病变中。
