Praznik W, Spies T
Institut für Chemie, Universität für Bodenkultur, Wien, Austria.
Carbohydr Res. 1993 Apr 23;243(1):91-7. doi: 10.1016/0008-6215(93)84083-i.
Fructo-oligosaccharides from red squill (Urginea maritima) were isolated by precipitation with methanol, GPC on Biogel P2/P4, and reversed-phase HPLC. Structures of the tri- and tetra-saccharides were verified by the reductive cleavage method. A tetrasaccharide that contained both (2-->1)- and (2-->6)-linked beta-D-Fru f residues was isolated. The higher fractions from GPC were analysed by the reductive cleavage method without prior purification by reversed-phase HPLC. The mode of biosynthesis of sinistrin is discussed.
通过甲醇沉淀、在Biogel P2/P4上进行凝胶渗透色谱（GPC）以及反相高效液相色谱（HPLC），从红海葱（海葱属）中分离出低聚果糖。通过还原裂解方法验证了三糖和四糖的结构。分离出一种同时含有（2→1）-和（2→6）-连接的β-D-呋喃果糖残基的四糖。GPC的较高馏分在未经反相HPLC预先纯化的情况下通过还原裂解方法进行分析。讨论了左聚糖的生物合成模式。
