Sims Ian M
Industrial Research Limited, PO Box 31-310, Lower Hutt, New Zealand.
Phytochemistry. 2003 Jun;63(3):351-9. doi: 10.1016/s0031-9422(03)00132-8.
The water-soluble carbohydrates (WSCs) extracted from the underground parts of Arthropodium cirratum, Astelia banksii, Bulbinella hookeri, Dianella nigra and Xeronema callistemon, and the flower stem of Phormium tenax have been investigated. Extracts of A. cirratum, B. hookeri, D. nigra and P. tenax contained 108.4, 28.1, 41.9 and 29.7 mg gFW(-1) WSCs, respectively. Thin-layer chromatography (TLC) showed that these species contained fructans. Extracts of A. banksii and X. callistemon each contained 11 mg gFW(-1) WSCs or less, and TLC detected only monosaccharides and sucrose. Reverse-phase (RP) HPLC and glycosyl linkage analysis showed that extracts of B. hookeri contained predominantly a linear series of fructans, with 1-linked and terminal fructofuranosyl (Fruf) residues and terminal glucopyranose (Glcp). RP-HPLC of extracts of A. cirratum, D. nigra and P. tenax showed a more complex pattern of oligosaccharides. Linkage analysis showed that these extracts contained fructans with 1-linked Fruf, 6-linked Fruf and 1,6-branched Fruf. Extracts of D. nigra and P. tenax contained 6-Glcp with only trace amounts of terminal Glcp, while A. cirratum contained mostly terminal Glcp with a trace of 6-Glcp. Differences in fructan and fructo-oligosaccharide structure between species shown in this study, together with published data, suggests that there are differences in the activities of the various fructosyltransferases responsible for biosynthesis of fructans in species within the Asparagales.
