Vander Heide R S, Reimer K A, Jennings R B
Department of Pathology, Duke University Medical Center, Durham, NC 27710.
Cardiovasc Res. 1993 Apr;27(4):669-73. doi: 10.1093/cvr/27.4.669.
Studies in rabbits suggest that the cardioprotective effects of adenosine against lethal cell injury may be related to production of adenosine and subsequent activation of adenosine A1 receptors. However, it is not known whether intracoronary adenosine therapy can mimic the metabolic sparing effects of preconditioning in rabbits or dogs. The purpose of this study was to determine the effect of intracoronary adenosine on ischaemic metabolism in totally ischaemic canine myocardium.
Dog hearts (n = 13) were excised and the coronary arteries were perfused with an oxygenated Krebs' buffer containing glucose. Adenosine was added to the buffer perfusing the circumflex (treated) region. Following perfusion, control and treated beds from each heart were subjected to 90 min total ischaemia at 37 degrees C. Tissue levels of ATP and glycolytic intermediates were determined at several time points during the ischaemic incubation.
Adenosine significantly slowed the rate of ATP depletion, glycogen utilisation, and lactate accumulation during the first 20 minutes of total ischaemia.
The results suggest that adenosine is capable of slowing ischaemic metabolism and they are consistent with the hypothesis that adenosine may mediate ischaemic preconditioning.
对兔子的研究表明，腺苷对致死性细胞损伤的心脏保护作用可能与腺苷的产生及随后腺苷A1受体的激活有关。然而，冠状动脉内腺苷治疗是否能模拟兔子或狗预处理的代谢节省效应尚不清楚。本研究的目的是确定冠状动脉内腺苷对完全缺血犬心肌缺血代谢的影响。
切除犬心脏（n = 13），用含葡萄糖的含氧 Krebs 缓冲液灌注冠状动脉。将腺苷添加到灌注回旋支（处理）区域的缓冲液中。灌注后，将每颗心脏的对照和处理区域在37℃下进行90分钟的完全缺血。在缺血孵育期间的几个时间点测定组织中ATP和糖酵解中间产物的水平。
在完全缺血的前20分钟内，腺苷显著减慢了ATP消耗、糖原利用和乳酸积累的速率。
结果表明腺苷能够减缓缺血代谢，这与腺苷可能介导缺血预处理的假说一致。
