Braw-Tal R, Bor A, Gootwine E
Volcani Center, Institute of Animal Science, Bet Dagan, Israel.
Domest Anim Endocrinol. 1993 Apr;10(2):87-94. doi: 10.1016/0739-7240(93)90014-3.
Plasma immunoreactive inhibin (i-inhibin) was measured in Assaf and Booroola-Assaf (1/2 Booroola-1/2 Assaf) ewe lambs between the ages of 2 and 15 weeks and correlated with plasma FSH. I-inhibin concentrations were similar in both genotypes and was maximal at 2 weeks of life and decreased thereafter. These levels (mean +/- s.e.) were 1.28 +/- 0.12 and 1.14 +/- 0.10 ng/ml at 2 weeks and 0.55 +/- 0.06 and 0.45 +/- 0.05 ng/ml at 15 weeks of age in Assaf (n = 13) and Booroola-Assaf (n = 13) respectively. In Assaf ewes an inverse correlation between plasma inhibin and FSH was observed at 4 and 15 weeks but not at 2 weeks of age. In Booroola-Assaf ewes, no significant correlation was found at any age studied. At 4 weeks of age, Assaf and Booroola-Assaf ewe lambs had very different ovarian morphology. Assaf lambs had large ovaries with a mean diameter of 1.00 +/- 0.09 cm (mean +/- s.e.) and many preantral and antral follicles present, while Booroola-Assaf had much smaller ovaries (0.66 +/- 0.03 cm, p < 0.05) with only a few antral follicles. It seems likely that the FecB allele from the Boorola Merino influenced the structure of the ovary although the contribution of other Booroola genes cannot be ruled out. In summary, the plasma i-inhibin concentrations decreased between 2 and 15 weeks of age irrespective of genotype. It is speculated that in prepubertal ewe lambs the ratio of bioactive to immunoreactive inhibin varies with age and between the genotypes with different ovarian development.
在2至15周龄的阿萨夫羊和布鲁拉-阿萨夫羊（1/2布鲁拉-1/2阿萨夫）母羊羔羊中测定了血浆免疫反应性抑制素（i-抑制素），并将其与血浆促卵泡素（FSH）进行关联分析。两种基因型的i-抑制素浓度相似，在出生后2周时达到最高，随后下降。在2周龄时，阿萨夫羊（n = 13）和布鲁拉-阿萨夫羊（n = 13）的这些水平（平均值±标准误）分别为1.28±0.12和1.14±0.10 ng/ml；在15周龄时分别为0.55±0.06和0.45±0.05 ng/ml。在阿萨夫母羊中，在4周和15周龄时观察到血浆抑制素与FSH呈负相关，但在2周龄时未观察到。在布鲁拉-阿萨夫母羊中，在所研究的任何年龄均未发现显著相关性。在4周龄时，阿萨夫羊和布鲁拉-阿萨夫羊母羊羔羊的卵巢形态有很大差异。阿萨夫羊羔羊的卵巢较大，平均直径为1.00±0.09 cm（平均值±标准误），有许多腔前卵泡和腔卵泡，而布鲁拉-阿萨夫羊的卵巢要小得多（0.66±0.03 cm，p < 0.05），只有少数腔卵泡。虽然不能排除其他布鲁拉基因的作用，但似乎布鲁拉美利奴羊的FecB等位基因影响了卵巢结构。总之，无论基因型如何，血浆i-抑制素浓度在2至15周龄之间都会下降。据推测，在青春期前的母羊羔羊中，生物活性抑制素与免疫反应性抑制素的比例随年龄以及卵巢发育不同的基因型而变化。
