Nakagawa S, Hara M, Seki M, Yagita H, Tagami H, Aiba S
Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan.
Eur J Immunol. 1993 Jul;23(7):1705-10. doi: 10.1002/eji.1830230746.
It has been reported that Thy-1+CD3+CD4-CD8- cells as well as Langerhans cells migrate from organ-cultured murine skin into culture medium. We examined whether these Thy-1+ populations of migrating cells were derived from Thy-1+ dendritic epidermal T cells (Thy-1+ DEC) and found that they were Thy-1+CD3+CD4-CD8-gamma/delta TcR+ (gamma delta+T) cells but did not express V gamma 5TcR, which was used by a vast majority of Thy-1+ DEC. Recently, a unique interaction between stromal cells and lymphohemopoietic progenitors has been reported in bone marrow and thymus. In this study, we established fibroblastoid cutaneous stromal cell (CSC) lines and clones from murine skin and examined the interaction between CSC and gamma delta+T cells. When these gamma delta+T cells were co-cultured with CSC, a marked proliferation of small lymphoid cells was observed only in the presence of interleukin (IL)-2. Neither CSC alone nor IL-2 alone could induce a similar proliferation. Flow cytometry revealed that they were Thy-1+CD3+CD4-CD8-gamma/delta TcR+ but V gamma 5TcR-. Analysis of the major segments of their TcR by polymerase chain reaction demonstrated that V gamma 1, V gamma 2, V gamma 4 and all of the V delta chains from V delta 1 to V delta 7 were used without any predominant pattern. These data indicate the possible presence of gamma/delta+T cells other than V gamma 5TcR+Thy-1+ DEC in the murine skin and the unique capacity of the CSC to support the growth of these migrating gamma/delta+ T cells. The nomenclature of murine T cell receptor gamma chain is according to Reilly et al. (Nature 1986. 321:878). The relationship between the different nomenclature systems is summarized in Takagi et al. (J. Immunol. 1989. 141:2112).
据报道，Thy-1⁺CD3⁺CD4⁻CD8⁻细胞以及朗格汉斯细胞从器官培养的小鼠皮肤迁移至培养基中。我们检测了这些迁移细胞的Thy-1⁺群体是否源自Thy-1⁺树突状表皮T细胞（Thy-1⁺DEC），结果发现它们是Thy-1⁺CD3⁺CD4⁻CD8⁻γ/δTcR⁺（γδ⁺T）细胞，但不表达Vγ5TcR，而绝大多数Thy-1⁺DEC会表达该受体。最近，在骨髓和胸腺中报道了基质细胞与淋巴细胞造血祖细胞之间存在独特的相互作用。在本研究中，我们从小鼠皮肤建立了成纤维细胞样皮肤基质细胞（CSC）系和克隆，并检测了CSC与γδ⁺T细胞之间的相互作用。当这些γδ⁺T细胞与CSC共培养时，仅在存在白细胞介素（IL）-2的情况下观察到小淋巴细胞显著增殖。单独的CSC或单独的IL-2均不能诱导类似的增殖。流式细胞术显示它们是Thy-1⁺CD3⁺CD4⁻CD8⁻γ/δTcR⁺但Vγ5TcR⁻。通过聚合酶链反应对其TcR的主要片段进行分析表明，使用了Vγ1、Vγ2、Vγ4以及从Vδ1到Vδ7的所有Vδ链，没有任何优势模式。这些数据表明在小鼠皮肤中可能存在除Vγ5TcR⁺Thy-1⁺DEC之外的γδ⁺T细胞，以及CSC支持这些迁移的γδ⁺T细胞生长的独特能力。小鼠T细胞受体γ链的命名遵循Reilly等人（《自然》1986年。321:878）的方法。不同命名系统之间的关系总结于Takagi等人（《免疫学杂志》1989年。141:2112）。
