Ota Y, Kobata T, Seki M, Yagita H, Shimada S, Huang Y Y, Takagaki Y, Okumura K
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Eur J Immunol. 1992 Feb;22(2):595-8. doi: 10.1002/eji.1830220245.
The dendritic epidermal cells (dEC) from young (8-week-old) athymic mice were found to express mRNA for gamma/delta T cell receptors (TcR). The mRNA found in the dEC from athymic mice was expressed only for V gamma 1-J gamma 4-C gamma 4 and V delta 6-J delta 1-C delta with both chains having productive rearrangements and junctional diversity. On the other hand, the dEC from euthymic mice carried V gamma 5-J gamma 1-C gamma 1 and V delta 1-J delta 2-C delta in addition to the V gamma 1/V delta 6 subset found in athymic mice. Therefore, this V gamma 1/V delta 6 subset, which has been implicated in the murine gamma/delta T cell responses to mycobacterial antigens and self heat-shock proteins, is the product of extrathymic maturation in the dEC from both athymic and euthymic mice.
研究发现，来自幼年（8周龄）无胸腺小鼠的树突状表皮细胞（dEC）可表达γ/δT细胞受体（TcR）的mRNA。在无胸腺小鼠的dEC中发现的mRNA仅表达Vγ1-Jγ4-Cγ4和Vδ6-Jδ1-Cδ，两条链均具有有效的重排和连接多样性。另一方面，除了在无胸腺小鼠中发现的Vγ1/Vδ6亚群外，来自有胸腺小鼠的dEC还携带Vγ5-Jγ1-Cγ1和Vδ1-Jδ2-Cδ。因此，这个与小鼠γ/δT细胞对分枝杆菌抗原和自身热休克蛋白的反应有关的Vγ1/Vδ6亚群，是无胸腺和有胸腺小鼠dEC中胸腺外成熟的产物。
