共翻译过程中血红素与新生珠蛋白链的结合。
Cotranslational heme binding to nascent globin chains.
作者信息
Komar A A, Kommer A, Krasheninnikov I A, Spirin A S
机构信息
Department of Molecular Biology, Faculty of Biology, Moscow State University, Russian Federation.
出版信息
FEBS Lett. 1993 Jul 12;326(1-3):261-3. doi: 10.1016/0014-5793(93)81803-8.
Globin synthesis in cell-free extracts of rabbit reticulocytes was carried out in the presence of 3H-labeled hemin. Sucrose gradient centrifugation analysis revealed [3H]hemin in the polyribosome fraction. The addition of puromycin resulted in the release of both [3H]hemin- and [14C]leucine-labeled polypeptide from the polyribosomes. The data suggest cotranslational folding of the globin molecule on the ribosome and cotranslational heme binding to the nascent globin chain.
在含有³H标记血红素的情况下，对兔网织红细胞的无细胞提取物进行珠蛋白合成。蔗糖梯度离心分析显示多核糖体组分中存在[³H]血红素。加入嘌呤霉素会导致多核糖体释放出[³H]血红素和[¹⁴C]亮氨酸标记的多肽。这些数据表明珠蛋白分子在核糖体上进行共翻译折叠，且血红素在新生珠蛋白链上进行共翻译结合。
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