Matsumura K, Shasby D M, Campbell K P
Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242.
FEBS Lett. 1993 Jul 12;326(1-3):289-93. doi: 10.1016/0014-5793(93)81810-m.
Dystrophin-related protein (DRP or utrophin) is an autosomal homologue of dystrophin, a membrane cytoskeletal protein encoded by the Duchenne muscular dystrophy gene. In contrast to dystrophin which is predominantly expressed in muscle, DRP is expressed in various tissues. Here we report the purification and biochemical characterization of DRP from lung which shows the highest levels of DRP expression among adult tissues. DRP was purified from the high alkaline extract of lung membranes using heparin-agarose column chromatography followed by anti-DRP immunoaffinity column chromatography. DRP was expressed in the cultured pulmonary artery endothelial cells. Expression of DRP in endothelial cells could explain its abundant expression in lung. In analogy to dystrophin of muscle cells, DRP could be playing an important role in the mechanical stress mechanisms of endothelial cells.
抗肌萎缩蛋白相关蛋白（DRP或抗肌萎缩蛋白聚糖）是抗肌萎缩蛋白的常染色体同源物，抗肌萎缩蛋白是由杜氏肌营养不良基因编码的一种膜细胞骨架蛋白。与主要在肌肉中表达的抗肌萎缩蛋白不同，DRP在各种组织中均有表达。在此，我们报告了从肺中纯化DRP及其生化特性，肺是成年组织中DRP表达水平最高的器官。使用肝素-琼脂糖柱色谱法，随后进行抗DRP免疫亲和柱色谱法，从肺膜的高碱性提取物中纯化出DRP。DRP在培养的肺动脉内皮细胞中表达。DRP在内皮细胞中的表达可以解释其在肺中的丰富表达。类似于肌肉细胞中的抗肌萎缩蛋白，DRP可能在内皮细胞的机械应激机制中发挥重要作用。
