Cooper E L, Leung M K, Suzuki M M, Vick K, Cadet P, Stefano G B
Department of Anatomy and Cell Biology, School of Medicine, University of California, Los Angeles 90024.
Dev Comp Immunol. 1993 May-Jun;17(3):201-9. doi: 10.1016/0145-305x(93)90039-s.
Substances that were immunoreactive in an RIA specific for met-enkephalin were detected following HPLC fractionation of earthworm coelomic fluid. Earthworm coelomocytes and human granulocytes were analyzed for changes in conformation based on measurements of cellular area and perimeter and expressed mathematically by using the Form Factor (FF). For coelomocytes the FF decreased following exposure to DAMA, a synthetic enkephalin analogue (D-Ala2, Met5-enkephalinamide). DAMA stimulated migration whereas untreated cells and those exposed to the specific opiate blocker naloxone did not move. The enkephalin-like molecule when exposed to human granulocytes stimulated an increased number of activated cells. Our results suggest a relationship between the immune and nervous systems of earthworms.
对蚯蚓体腔液进行高效液相色谱分级分离后，检测到在甲硫氨酸脑啡肽特异性放射免疫分析中具有免疫反应性的物质。基于细胞面积和周长的测量，分析了蚯蚓体腔细胞和人类粒细胞的构象变化，并通过形状因子（FF）进行数学表达。对于体腔细胞，在暴露于合成脑啡肽类似物DAMA（D-丙氨酸2，甲硫氨酸5-脑啡肽酰胺）后，FF降低。DAMA刺激迁移，而未处理的细胞和暴露于特异性阿片受体阻断剂纳洛酮的细胞则不移动。当暴露于人类粒细胞时，脑啡肽样分子刺激活化细胞数量增加。我们的结果表明蚯蚓的免疫系统和神经系统之间存在关联。
